| Autor: |
Soares Júnior FL; Laboratório de Microbiologia Ambiental, Embrapa, Jaguariúna, SP, Brazil. ; Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP, Brazil., Dias AC; Laboratório de Microbiologia Ambiental, Embrapa, Jaguariúna, SP, Brazil. ; Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP, Brazil., Fasanella CC; Departamento de Ciência do Solo, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba, SP, Brazil., Taketani RG; Laboratório de Microbiologia Ambiental, Embrapa, Jaguariúna, SP, Brazil., de Souza Lima AO; Laboratório de Microbiologia Aplicada, Universidade do Vale do Itajaí, Itajaí, SC, Brazil., Melo IS; Laboratório de Microbiologia Ambiental, Embrapa, Jaguariúna, SP, Brazil., Andreote FD; Departamento de Ciência do Solo, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba, SP, Brazil. |
| Abstrakt: |
The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from a mangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria, enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplemented with 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33 isolates) than exoglycolytic (19 isolates), and the degradation activity was shown to be modulated by the presence of NaCl. The isolated bacteria were clustered by BOX-PCR and further classified on the basis of partial 16S rRNA sequences as Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes or Bacteroidetes. Therefore, this study highlights the importance of studies focusing on the endemic species found in mangroves to exploit them as novel biotechnological tools for the degradation of cellulose. |
