| Autor: |
Grigoras I; Institut des Sciences du Végétal, CNRS, 91198 Gif sur Yvette, France., Ginzo AIDC; Departamento de Protección Vegetal, Instituto Nacional de Investigación y Tecnología Agraria (INIA), Carretera de La Coruna Km. 7.0, Madrid 28040, Spain., Martin DP; Computational Biology Group, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa., Varsani A; Electron Microscope Unit, University of Cape Town, Rondebosch, 7701, Cape Town, South Africa.; Department of Plant Pathology and Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA.; School of Biological Sciences and Biomolecular Interaction Centre, University of Canterbury, Christchurch, 8140, New Zealand., Romero J; Departamento de Protección Vegetal, Instituto Nacional de Investigación y Tecnología Agraria (INIA), Carretera de La Coruna Km. 7.0, Madrid 28040, Spain., Mammadov AC; Department of Fundamental Problems of Biological Productivity, Institute of Botany, Azerbaijan National Academy of Sciences, 40 Badamdar Highway, Baku AZ 1073, Azerbaijan., Huseynova IM; Department of Fundamental Problems of Biological Productivity, Institute of Botany, Azerbaijan National Academy of Sciences, 40 Badamdar Highway, Baku AZ 1073, Azerbaijan., Aliyev JA; Department of Fundamental Problems of Biological Productivity, Institute of Botany, Azerbaijan National Academy of Sciences, 40 Badamdar Highway, Baku AZ 1073, Azerbaijan., Kheyr-Pour A; 31 Allée des Joncherettes, 91190 Gif sur Yvette, France., Huss H; Lehr- und Forschungszentrum für Landwirtschaft (LFZ) Raumberg-Gumpenstein, Versuchsstation Lambach/Stadl-Paura, 4651 Stadl-Paura, Austria., Ziebell H; Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, 38104 Braunschweig, Germany., Timchenko T; Institut des Sciences du Végétal, CNRS, 91198 Gif sur Yvette, France., Vetten HJ; Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, 38104 Braunschweig, Germany., Gronenborn B; Institut des Sciences du Végétal, CNRS, 91198 Gif sur Yvette, France. |
| Abstrakt: |
The recent identification of a new nanovirus, pea necrotic yellow dwarf virus, from pea in Germany prompted us to survey wild and cultivated legumes for nanovirus infections in several European countries. This led to the identification of two new nanoviruses: black medic leaf roll virus (BMLRV) and pea yellow stunt virus (PYSV), each considered a putative new species. The complete genomes of a PYSV isolate from Austria and three BMLRV isolates from Austria, Azerbaijan and Sweden were sequenced. In addition, the genomes of five isolates of faba bean necrotic yellows virus (FBNYV) from Azerbaijan and Spain and those of four faba bean necrotic stunt virus (FBNSV) isolates from Azerbaijan were completely sequenced, leading to the first identification of FBNSV occurring in Europe. Sequence analyses uncovered evolutionary relationships, extensive reassortment and potential remnants of mixed nanovirus infections, as well as intra- and intercomponent recombination events within the nanovirus genomes. In some virus isolates, diverse types of the same genome component (paralogues) were observed, a type of genome complexity not described previously for any member of the family Nanoviridae. Moreover, infectious and aphid-transmissible nanoviruses from cloned genomic DNAs of FBNYV and BMLRV were reconstituted that, for the first time, allow experimental reassortments for studying the genome functions and evolution of these nanoviruses. |
