[Effect of human umbilici mesenchymal stromal cells implantation on the BDNF expression in diabetic foot rats].

Autor: Zhou N; Institute of Neuroscience, Kunming Medical University, Kunming 650031, China., Wang QP; Institute of Neurological Disease and Translation Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China., Jin XF; Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China., Hou ZL; Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China., Peng BK; Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China., Dan QQ; Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China., Wang TH; Institute of Neuroscience, Kunming Medical University, Kunming 650031, China.
Jazyk: čínština
Zdroj: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2013 Nov; Vol. 44 (6), pp. 931-4.
Abstrakt: Unlabelled: OBJECTITVE: To investigate the effect of human umbilici mesenchymal cells (HUMSCs) implantation on the brain derived neurotrophic factor (BDNF) expression in diabetic foot rats.
Methods: SD rats were divided into three groups (n = 12): normal group, diadetic foot model group and HUMSC treatment group. Diabetic foot model in rats was established, then prepared HUMSC were implanted on the diabetic foot ulcers in rats, and control ones were administrated with saline only. The area of ulceration, sensory function, BDNF expression and its localization were determined by using morphology, physiological function measurement, RT-PCR and immunohistochemistry assay.
Result: Siglificantly decreased area of ulceration in diabetic foot rats of HUMSC implantation group was observed. This was simultaneously companied with the sensory function improvement (P < 0.05). RT-PCR showed that BDNF mRNA expression was significantly up regulated (P < 0.05). BDNF immunstaining was located in epithelia tissue and the protein level of BDNF was markedly increased (P < 0.05).
Conclusion: HUMSC implantation maybe an effective strategy on the treatment of ulceration in diabetic foot rats, and the possible mechanism may involve in BDNF expression.
Databáze: MEDLINE
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