A rapid method for determining soot content of lungs in diesel-exposed rodents.

Autor: Henderson RF; Lovelace Biomedical and Environmental Research Institute, Inhalation Toxicology Research Institute, Albuquerque, NM 87185., Waide JJ, Mauderly JL, McClellan RO
Jazyk: angličtina
Zdroj: Journal of applied toxicology : JAT [J Appl Toxicol] 1987 Dec; Vol. 7 (6), pp. 357-60.
DOI: 10.1002/jat.2550070602
Abstrakt: A rapid optical method for determining the quantity of soot in the lungs of rodents exposed to diluted diesel exhaust has been developed. The method is nondestructive to enzymatic components of the lung, allowing dose and biochemical assays to be done on the same sample. The lung burdens of soot were determined by comparing the extinction of light at 620 nm of lung homogenates from exposed animals to that of lung homogenates containing spiked standards.
Databáze: MEDLINE