| Autor: |
Sheng HZ; Department of Psychology, School of Agriculture, La Trobe University, Bundoora, Vic., Australia., Martenson RE, Grgacic EV, Dowse CA, Carnegie RL, Bernard CC |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of neuroimmunology [J Neuroimmunol] 1988 Feb; Vol. 17 (3), pp. 183-92. |
| DOI: |
10.1016/0165-5728(88)90066-5 |
| Abstrakt: |
A rapid and sensitive method for the identification of antigenic determinants recognised by monoclonal and polyclonal antibodies directed against myelin basic protein (MBP) is described. By electroimmunoblotting a series of overlapping peptides covering the entire MBP molecule with monoclonal anti-MBP antibodies, the binding pattern of immunoreactive peptides can be rapidly determined and the reactive antigenic determinant identified. This procedure, which can be performed with both native and synthetic peptides, can also with appropriate modification, be applied to the analysis of naturally occurring or experimentally induced polyclonal anti-MBP autoantibodies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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