Evaluation of non-conventional polar modifiers on immobilized chiral stationary phases for improved resolution of enantiomers by supercritical fluid chromatography.

Autor: DaSilva JO; Merck Research Laboratories, Rahway, NJ 07065, USA. Electronic address: jimmy.dasilva@merck.com., Coes B; Merck Research Laboratories, Rahway, NJ 07065, USA., Frey L; Merck Research Laboratories, Rahway, NJ 07065, USA., Mergelsberg I; Merck Research Laboratories, Rahway, NJ 07065, USA., McClain R; Merck Research Laboratories, West Point, PA 19486, USA., Nogle L; Merck Research Laboratories, Boston, MA 02115, USA., Welch CJ; Merck Research Laboratories, Rahway, NJ 07065, USA.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2014 Feb 07; Vol. 1328, pp. 98-103. Date of Electronic Publication: 2013 Dec 31.
DOI: 10.1016/j.chroma.2013.12.073
Abstrakt: An evaluation of the use of non-conventional polar modifiers for the supercritical fluid chromatographic separation of enantiomers on immobilized chiral stationary phases is presented. The resolution of a group of nine commercially available racemates is studied on the Chiralpak IA, IB, IC, ID, IE, and IF chiral stationary phases using CO2-based eluents containing non-conventional polar modifiers such as dichloromethane, chloroform, tetrahydrofuran, 2-methyl tetrahydrofuran, methyl tert-butyl ether, cyclopentyl methyl ether, acetone, ethyl acetate, toluene, 2,2,2-trifluoroethanol, and N,N-dimethylformamide. Screening experiments and method development for the commercial racemates on the immobilized columns with the non-conventional solvents demonstrated an ability to adjust the retention and improve resolution. From these results we were able to assign a general eluotropic relationship between the non-conventional solvents and methanol. A general ability to selectively adjust chromatographic retention while improving analyte solubility can lead to improved preparative chromatographic performance.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: