A microfluidic-enabled mechanical microcompressor for the immobilization of live single- and multi-cellular specimens.

Autor: Yan Y; Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA., Jiang L; Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA., Aufderheide KJ; Department of Biology, Texas A&M University, College Station, TX 77843, USA., Wright GA; Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA., Terekhov A; Center for Laser Applications, University of Tennessee Space Institute, Tullahoma, TN 37388, USA., Costa L; Center for Laser Applications, University of Tennessee Space Institute, Tullahoma, TN 37388, USA., Qin K; Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA., McCleery WT; Department of Physics and Astronomy, Vanderbilt University, Nashville, TN 37232, USA., Fellenstein JJ; Vanderbilt Machine Shop, Vanderbilt University, Nashville, TN 37232, USA., Ustione A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA., Robertson JB; Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA., Johnson CH; Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA., Piston DW; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA., Hutson MS; Department of Physics and Astronomy, Vanderbilt University, Nashville, TN 37232, USA., Wikswo JP; Department of Physics and Astronomy, Vanderbilt University, Nashville, TN 37232, USA., Hofmeister W; Center for Laser Applications, University of Tennessee Space Institute, Tullahoma, TN 37388, USA., Janetopoulos C; Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA.
Jazyk: angličtina
Zdroj: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada [Microsc Microanal] 2014 Feb; Vol. 20 (1), pp. 141-51. Date of Electronic Publication: 2014 Jan 21.
DOI: 10.1017/S1431927613014037
Abstrakt: A microcompressor is a precision mechanical device that flattens and immobilizes living cells and small organisms for optical microscopy, allowing enhanced visualization of sub-cellular structures and organelles. We have developed an easily fabricated device, which can be equipped with microfluidics, permitting the addition of media or chemicals during observation. This device can be used on both upright and inverted microscopes. The apparatus permits micrometer precision flattening for nondestructive immobilization of specimens as small as a bacterium, while also accommodating larger specimens, such as Caenorhabditis elegans, for long-term observations. The compressor mount is removable and allows easy specimen addition and recovery for later observation. Several customized specimen beds can be incorporated into the base. To demonstrate the capabilities of the device, we have imaged numerous cellular events in several protozoan species, in yeast cells, and in Drosophila melanogaster embryos. We have been able to document previously unreported events, and also perform photobleaching experiments, in conjugating Tetrahymena thermophila.
Databáze: MEDLINE
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