Analysis of differentially expressed genes in colorectal adenocarcinoma with versus without metastasis by three-dimensional oligonucleotide microarray.

Autor:	Moura Franco RM; UNIFESP - EPM (Federal University Of Sao Paulo), Escola Paulista de Medicina São Paulo, Brasil., Linhares MM; UNIFESP - EPM (Federal University Of Sao Paulo), Escola Paulista de Medicina São Paulo, Brasil., Lustosa SS; UNIFESP - EPM (Federal University Of Sao Paulo), Escola Paulista de Medicina São Paulo, Brasil., Silva ID; UNIFESP - EPM (Federal University Of Sao Paulo), Escola Paulista de Medicina São Paulo, Brasil., Souza NC; Piu XII Foundation - Barretos' Cancer Hospital São Paulo, Brasil., Matos D; UNIFESP - EPM (Federal University Of Sao Paulo), Escola Paulista de Medicina São Paulo, Brasil.
Jazyk:	angličtina
Zdroj:	International journal of clinical and experimental pathology [Int J Clin Exp Pathol] 2013 Dec 15; Vol. 7 (1), pp. 255-63. Date of Electronic Publication: 2013 Dec 15 (Print Publication: 2014).
Abstrakt:	Background: Our objective was to examine how the gene expression profile of tumor tissue correlates with lymph node metastasis in patients with advanced colorectal adenocarcinoma (CRAC). Methods: We studied 36 patients (20 men and 16 women, 22-90 years of age) treated for CRAC (classifications of T2, T3, or T4; histological grade of G1 or G2). Amplified tumor mRNA samples were exposed to 20,000 human sequence probes and digitized images of the hybridized samples were analyzed. Results: On average, 2389 probes were detected above the background, with an average correlation R value of 0.19 between data from different patient groups (with or without lymph node invasion, colon or rectal, with or without angio-lymphatic invasion, with or without recurrence). Lymph node metastasis had a statistically significant signature according to Significance Analysis of Microarrays (SAM) and parametric t-tests, with a false discovery rate (FDR)=0.1% and p=0.001, respectively. Cross-correlation of these two tests identified 102 transcripts as being potentially related to node metastases, with fold changes in the range of 2.182-12.960. Conclusion: We identified 102 differentially expressed genes related to the presence of lymph node metastases in patients with advanced colorectal cancer.
Databáze:	MEDLINE
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