| Autor: |
Tinh NT; Research Institute for Aquaculture No. 2, 116 Nguyen Dinh Chieu, District 1, Ho Chi Minh City, Vietnam., Dung NV; Research Institute for Aquaculture No. 2, 116 Nguyen Dinh Chieu, District 1, Ho Chi Minh City, Vietnam., Trung CT; Research Institute for Aquaculture No. 2, 116 Nguyen Dinh Chieu, District 1, Ho Chi Minh City, Vietnam., Thuy VT; Research Institute for Aquaculture No. 2, 116 Nguyen Dinh Chieu, District 1, Ho Chi Minh City, Vietnam. |
| Abstrakt: |
aiiA gene encoding AHL-lactonase was isolated from Bacillus cereus strain N26.2, originating from a striped catfish (Pangasianodon hypophthalmus) pond in Vietnam. This gene, abbreviated as aiiA(N26.2), was cloned and expressed in a competent Escherichia coli strain BL21(DE3)pLysS. The resulting protein, abbreviated as AiiAN26.2, was highly active in the pH range of 6-8 and could retain 80 % of the maximum activity under storage for 5 days at 4 °C or for 3 days at 20 °C. These properties of AiiAN26.2 protein confers its future application via feed supplementation, with the purpose of controlling aquaculture pathogens which regulate the virulence via a quorum sensing system. |
