HTLV-1 infects human mesenchymal stromal cell in vitro and modifies their phenotypic characteristics.

Autor: Rodrigues ES; Regional Blood Center of Ribeirão Preto, University of São Paulo, Brazil; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil., de Macedo MD; Regional Blood Center of Ribeirão Preto, University of São Paulo, Brazil; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil., Pinto MT; Regional Blood Center of Ribeirão Preto, University of São Paulo, Brazil; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil., Orellana MD; Regional Blood Center of Ribeirão Preto, University of São Paulo, Brazil; School of Medicine of Ribeirão Preto, University of São Paulo, Brazil., Rocha Junior MC; Regional Blood Center of Ribeirão Preto, University of São Paulo, Brazil; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil., de Magalhães DA; Regional Blood Center of Ribeirão Preto, University of São Paulo, Brazil., Tanaka Y; University of the Ryukyus, Okinawa, Japan., Takayanagui OM; School of Medicine of Ribeirão Preto, University of São Paulo, Brazil., Covas DT; Regional Blood Center of Ribeirão Preto, University of São Paulo, Brazil; School of Medicine of Ribeirão Preto, University of São Paulo, Brazil., Kashima S; Regional Blood Center of Ribeirão Preto, University of São Paulo, Brazil; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil. Electronic address: skashima@hemocentro.fmrp.usp.br.
Jazyk: angličtina
Zdroj: Virology [Virology] 2014 Jan 20; Vol. 449, pp. 190-9. Date of Electronic Publication: 2013 Dec 06.
DOI: 10.1016/j.virol.2013.11.022
Abstrakt: The typical characteristics of mesenchymal stem cells (MSCs) can be affected by inflammatory microenvironment; however, the exact contribution of HTLV-1 to MSC dysfunction remains to be elucidated. In this study, we demonstrated that MSC cell surface molecules VCAM-1 and ICAM-1 are upregulated by contact with HTLV-1, and HLA-DR was most highly expressed in MSCs co-cultured with MT2 cells. The expression levels of VCAM-1 and HLA-DR were increased in MSCs cultured in the presence of PBMCs isolated from HTLV-1-infected symptomatic individuals compared with those cultured with cells from asymptomatic infected individuals or healthy subjects. HTLV-1 does not impair the MSC differentiation process into osteocytes and adipocytes. In addition, MSCs were efficiently infected with HTLV-1 in vitro through direct contact with HTLV-1-infected cells; however, cell-free virus particles were not capable of causing infection. In summary, HTLV-1 can alter MSC function, and this mechanism may contribute to the pathogenesis of this viral infection.
(© 2013 Published by Elsevier Inc.)
Databáze: MEDLINE
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