Iridoid glycoside from the leaves of Clerodendrum volubile beauv. shows potent antioxidant activity against oxidative stress in rat brain and hepatic tissues.
| Autor: | Erukainure OL; 1Food Toxicology Laboratory, Food Technology Division, Federal Institute of Industrial Research, Lagos, Nigeria., Ebuehi OA, Choudhary IM, Adhikari A, Hafizur RM, Perveen S, Muhammad A, Elemo GN |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Journal of dietary supplements [J Diet Suppl] 2014 Mar; Vol. 11 (1), pp. 19-29. Date of Electronic Publication: 2014 Jan 10. |
| DOI: | 10.3109/19390211.2013.859213 |
| Abstrakt: | Aim: This study aims at reporting the isolation, structure elucidation, and antioxidant potentials of ajugoside from C. volubile leaves in sodium nitroprusside (SNP)-induced oxidative stressed rat brain and hepatic tissues. Materials and Method: An iridoid monoterpene, ajugoside was isolated from the n-butanol fraction of C. volubile and evaluated for its antioxidant protective potential on brain and liver tissues of male Wister rats in an ex vivo model. Two molar concentrations (6.4 × 10(-4) M and 1.28 × 10(-3) M) of the metabolite and SNP were incubated with the tissues homogenate at 37°C for 2 hr prior to the test and assayed for catalase, superoxide dismutase (SOD) activities, and lipid peroxidation. α tocopherol (6.4 × 10(-4) M) was used as standard. Results: Both molar concentrations exhibited high catalase activity in the tissues. However, 6.4 × 10(-4) M ajugoside exhibited a very high SOD activity (liver: 96.45 and brain: 96.30%) and inhibition of lipid peroxidation (liver: 88.11 and brain: 93.27%) compared to the standard. 1.28 × 10(-3) M ajugoside also exhibited good activities but lower than that of the standard and 6.4 × 10(-4) M ajugoside. Discussion and Conclusion: Ajugoside showed potent antioxidant activities as evidenced by the synergistic high activities of SOD and catalase as well as inhibition of lipid peroxidation in the studied tissues. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|