Evaluation of enrichment method for the detection of Vibrio cholerae O1 using a rapid dipstick test in Bangladesh.

Autor: George CM; Department of International Health Program in Global Disease Epidemiology and Control, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA., Rashid MU; International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh., Sack DA; Department of International Health Program in Global Disease Epidemiology and Control, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA., Bradley Sack R; Department of International Health Program in Global Disease Epidemiology and Control, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA., Saif-Ur-Rahman KM; International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh., Azman AS; Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA., Monira S; International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh., Bhuyian SI; International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh., Zillur Rahman KM; International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh., Toslim Mahmud M; International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh., Mustafiz M; International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh., Alam M; International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Jazyk: angličtina
Zdroj: Tropical medicine & international health : TM & IH [Trop Med Int Health] 2014 Mar; Vol. 19 (3), pp. 301-307. Date of Electronic Publication: 2014 Jan 09.
DOI: 10.1111/tmi.12252
Abstrakt: Background: To evaluate the specificity of the Crystal VC dipstick test for detecting cholera.
Methods: We compared direct testing using the Crystal VC dipstick test and testing after enrichment for 6 h in alkaline peptone water (APW) to bacterial culture as the gold standard. Samples positive by dipstick but negative by culture were also tested using PCR.
Results: Stool was collected from 125 patients. The overall specificities of the direct testing and testing after 6-h enrichment in APW compared to bacterial culture were 91.8% and 98.4% (P = 0.125), respectively, and the sensitivities were 65.6% and 75.0% (P = 0.07), respectively.
Conclusion: The increase in the sensitivity of the Crystal VC kit with the use of the 6-h enrichment step in APW compared to direct testing was marginally significant. The Crystal VC dipstick had a much higher specificity than previously reported (91-98%). Therefore, this method might be a promising screening tool for cholera outbreak surveillance in resource-limited settings where elimination of false-positive results is critical.
(© 2014 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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