Pentavalent single-domain antibodies reduce Campylobacter jejuni motility and colonization in chickens.

Autor: Riazi A; AbCelex Technologies Inc., Toronto, Ontario, Canada., Strong PC; Human Health Therapeutics, National Research Council of Canada, Ottawa, Ontario, Canada., Coleman R; Human Health Therapeutics, National Research Council of Canada, Ottawa, Ontario, Canada., Chen W; Human Health Therapeutics, National Research Council of Canada, Ottawa, Ontario, Canada., Hirama T; Human Health Therapeutics, National Research Council of Canada, Ottawa, Ontario, Canada., van Faassen H; Human Health Therapeutics, National Research Council of Canada, Ottawa, Ontario, Canada., Henry M; Dow AgroSciences, Indianapolis, Indiana, United States of America., Logan SM; Human Health Therapeutics, National Research Council of Canada, Ottawa, Ontario, Canada., Szymanski CM; Centennial Centre for Interdisciplinary Science, Department of Biological Sciences and Alberta Glycomics Centre, The University of Alberta, Edmonton, Alberta, Canada., Mackenzie R; Human Health Therapeutics, National Research Council of Canada, Ottawa, Ontario, Canada ; Centennial Centre for Interdisciplinary Science, Department of Biological Sciences and Alberta Glycomics Centre, The University of Alberta, Edmonton, Alberta, Canada., Ghahroudi MA; Human Health Therapeutics, National Research Council of Canada, Ottawa, Ontario, Canada ; School of Environmental Sciences, University of Guelph, Guelph, Ontario, Canada ; Department of Biology, Carleton University, Ottawa, Ontario, Canada.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2013 Dec 31; Vol. 8 (12), pp. e83928. Date of Electronic Publication: 2013 Dec 31 (Print Publication: 2013).
DOI: 10.1371/journal.pone.0083928
Abstrakt: Campylobacter jejuni is the leading cause of bacterial foodborne illness in the world, with symptoms ranging from acute diarrhea to severe neurological disorders. Contaminated poultry meat is a major source of C. jejuni infection, and therefore, strategies to reduce this organism in poultry, are expected to reduce the incidence of Campylobacter-associated diseases. We have investigated whether oral administration of C. jejuni-specific single-domain antibodies would reduce bacterial colonization levels in chickens. Llama single-domain antibodies specific for C. jejuni were isolated from a phage display library generated from the heavy chain IgG variable domain repertoire of a llama immunized with C. jejuni flagella. Two flagella-specific single-domain antibodies were pentamerized to yield high avidity antibodies capable of multivalent binding to the target antigen. When administered orally to C. jejuni-infected two-day old chicks, the pentabodies significantly reduced C. jejuni colonization in the ceca. In vitro, the motility of the bacteria was also reduced in the presence of the flagella-specific pentabodies, suggesting the mechanism of action is through either direct interference with flagellar motility or antibody-mediated aggregation. Fluorescent microscopy and Western blot analyses revealed specific binding of the anti-flagella pentabodies to the C. jejuni flagellin.
Databáze: MEDLINE
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