Expression profile of IL-35 mRNA in gingiva of chronic periodontitis and aggressive periodontitis patients: a semiquantitative RT-PCR study.

Autor: Kalburgi NB; Department of Periodontics, PMNM Dental College, Bagalkot, Karnataka 587101, India., Muley A; Department of Periodontics, PMNM Dental College, Bagalkot, Karnataka 587101, India., Shivaprasad BM; Department of Periodontics, Rajarajeswari Dental College and Hospital, Ramohalli Cross, Mysore Road, Bangalore, Karnataka 560060, India., Koregol AC; Department of Periodontics, PMNM Dental College, Bagalkot, Karnataka 587101, India.
Jazyk: angličtina
Zdroj: Disease markers [Dis Markers] 2013; Vol. 35 (6), pp. 819-23. Date of Electronic Publication: 2013 Nov 27.
DOI: 10.1155/2013/489648
Abstrakt: Background: Proinflammatory and anti-inflammatory cytokines play a key role in the pathogenesis of periodontal diseases. Secretion of bioactive IL-35 has been described by T regulatory cells (T(regs)) and is required for their maximal suppressive activity. T(regs) are involved in the modulation of local immune response in chronic periodontitis patients.
Objective: Hence, the present study was aimed to investigate the expression of IL-35 mRNA in chronic periodontitis and aggressive periodontitis patients.
Materials and Methods: The present study was carried out in 60 subjects, which included 20 chronic periodontitis patients, 20 aggressive periodontitis patients, and 20 periodontally healthy controls. IL-35 mRNA expression in gingival tissue samples of all subjects was semiquantitatively analyzed using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR).
Results: The present study demonstrated the expression of IL-35 mRNA in gingival tissues of all the three groups. IL-35 mRNA expression was highest in chronic periodontitis subjects (6.87 ± 2.32) as compared to the aggressive periodontitis group (4.71 ± 1.43) and least seen in healthy patients (3.03 ± 1.91).
Conclusion: The increased expression of IL-35 in chronic and aggressive periodontitis suggests its possible role in pathogenesis of periodontitis. Future studies done on large samples with intervention will strengthen our result.
Databáze: MEDLINE
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