Effect of pre-analytical conditions on the thrombodynamics assay.

Autor: Dashkevich NM; EA 4174, Université Lyon 1, Lyon, France; HemaCore LLC, Moscow, Russia; Center for Theoretical Problems of Physico-Chemical Pharmacology, Moscow, Russia,. Electronic address: dashkevichnm@gmail.com., Vuimo TA; Federal Research and Clinical Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia., Ovsepyan RA; Federal Research and Clinical Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia., Surov SS; Federal Research and Clinical Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia., Soshitova NP; HemaCore LLC, Moscow, Russia., Panteleev MA; HemaCore LLC, Moscow, Russia; Center for Theoretical Problems of Physico-Chemical Pharmacology, Moscow, Russia,; National Research Center for Hematology, Moscow, Russia; Physics Department, Moscow State University, Moscow, Russia., Ataullakhanov FI; HemaCore LLC, Moscow, Russia; Center for Theoretical Problems of Physico-Chemical Pharmacology, Moscow, Russia,; Federal Research and Clinical Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia; National Research Center for Hematology, Moscow, Russia; Physics Department, Moscow State University, Moscow, Russia., Negrier C; EA 4174, Université Lyon 1, Lyon, France; Unite d'Hemostase Clinique, Hopital Edouard Herriot, Lyon, France.
Jazyk: angličtina
Zdroj: Thrombosis research [Thromb Res] 2014 Mar; Vol. 133 (3), pp. 472-6. Date of Electronic Publication: 2013 Dec 14.
DOI: 10.1016/j.thromres.2013.12.014
Abstrakt: Introduction: Standardization of pre-analytical conditions is the obligatory step for all potential diagnostic tests. Spatial clot growth (Thrombodynamics) is a new global hemostasis assay that considers spatial organization of coagulation. The principal parameter is rate of fibrin clot growth from the tissue-factor coated surface. In this work we studied the pre-analytical variables of Thrombodynamics assay that include conditions of blood collection, sample preparation and storage.
Materials and Methods: Blood of apparently healthy volunteers was used. Eight types of citrate blood collection tubes were tested, centrifugation conditions for plasma preparation were evaluated and impact of plasma freezing/thawing was tested.
Results: Among the blood collection tubes tested, BD Vacutainer glass tubes showed a significantly higher clot growth rate compared to plastic tubes. There was no difference between 3.2% and 3.8% of sodium citrate. For plasma preparation, a single 15 min centrifugation at 1,600 g shows significantly increased clot growth rate compared to plasma obtained by two sequential centrifugations (15 min 1 600 g, 5min 10,000 g). There was no significant difference between 1,600 g and 2,100 g if the second centrifugation was performed. For the second centrifugation there was no difference between 20 min at 1 600 g and 5 min at 10,000g. Frozen-thawed plasma showed increased clot growth rate compared to fresh plasma.
Conclusion: The data represent the necessary steps for the standardization of Thrombodynamics assay and for the formulation of the operating guide.
(Copyright © 2013 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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