In vivo anti-inflammatory activity of Tabernaemontana divaricata leaf extract on male albino mice.

Autor: Jain S; College of Pharmacy, IPS Academy, Rajendra Nagar, AB Road, Indore MP India 452012. Electronic address: sachin225819@rediffmail.com., Sharma P; College of Pharmacy, IPS Academy, Rajendra Nagar, AB Road, Indore MP India 452012., Ghule S; College of Pharmacy, IPS Academy, Rajendra Nagar, AB Road, Indore MP India 452012., Jain A; College of Pharmacy, IPS Academy, Rajendra Nagar, AB Road, Indore MP India 452012., Jain N; Department of Pharmacy, Oriental University, Indore MP India 452001.
Jazyk: angličtina
Zdroj: Chinese journal of natural medicines [Chin J Nat Med] 2013 Sep; Vol. 11 (5), pp. 472-6.
DOI: 10.1016/S1875-5364(13)60086-2
Abstrakt: Aim: To study the in vivo anti-inflammatory activity of Tabernaemontana divaricata leaf extract on male albino mice.
Methods: Aqueous decoction and methanol leaf extracts were tested for their ability to reduce croton oil-induced edema in the mouse ear after topical application. The methanol leaf extract dose-dependently inhibited the croton oil-induced ear edema in mice (ID50 <500 μg·cm(-2)). A bioassay-guided liquid-liquid fractionation of this methanol extract gave four active fractions: water insoluble (F1), hexane (F2), ethyl acetate (F3) and water (F4).
Results: The hexane fraction showed a very high activity (42.1% inhibition at 0.7 μg·cm(-2)) as compared to the control. The other fractions were less active (F1: 56.1% at 506.2 μg·cm(-2); F3: 57.3% at 289.3 μg·cm(-2); and F4: 31.9% for 203.8 μg·cm(-2)) while indomethacin gave 48.8% of inhibition at 90 μg·cm(-2). The activity of F1 and F3 may be at least in part explained by the presence of anti-inflammatory flavonoids, while the activity was not correlated to the tannin contents. No compounds were detected in the most active F2 fraction.
Conclusions: The results give a rational support to the traditional use of T. divaricata in tropical India as anti-inflammatory agent.
(Copyright © 2013 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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