| Autor: |
Fernandes BF; Department of Ophthalmology and Pathology, The McGill University Health Center & Henry C. Witelson Ocular Pathology Laboratory, 3775 University St., Room 216, Montreal, QC, Canada, H3A 2B4, bruno.mtl@gmail.com., Coates J, Odashiro AN, Quezada C, Huynh A, Odashiro PR, Odashiro M, Burnier MN Jr |
| Jazyk: |
angličtina |
| Zdroj: |
Pathology oncology research : POR [Pathol Oncol Res] 2014 Jul; Vol. 20 (3), pp. 557-63. Date of Electronic Publication: 2013 Dec 14. |
| DOI: |
10.1007/s12253-013-9728-8 |
| Abstrakt: |
In order to better understand the role of HIF-1α in the proliferation of the retinoblastoma cells, a siRNA knockdown of HIF-1α followed by a proliferation assay was performed. Further sequencing was then carried out in order to assess knockdown efficiency and expression of HIF-1α. Upregulation of HIF-1α gene expression in CoCl2-treated retinoblastoma cells was demonstrated via melting curve analysis from PCR tests and was further analyzed using western blot and densitometry analysis. Reduction of HIF-1α expression in retinoblastoma, post HIF-1α knockdown, was observed after siRNA transfection into Y-79 cells. Knockdown of HIF-1α resulted in a significant decrease in proliferation thereby demonstrating that HIF-1α is involved in promoting survival and proliferation in retinoblastoma cells. Stabilization of HIF-1α in retinoblastoma cells using CoCl2 was unsuccessful. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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