Structure elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 33C, 33D, and revised structure of serotype 33B.

Autor: Lin FL; Pfizer Vaccine Research, 401 N. Middletown Road, Pearl River, NY 10965, USA. Electronic address: fiona.lin@pfizer.com., Vinogradov E; Institute for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa, ON K1A 0R6, Canada., Deng C; Pfizer Vaccine Research, 401 N. Middletown Road, Pearl River, NY 10965, USA., Zeller S; Pfizer Vaccine Research, 401 N. Middletown Road, Pearl River, NY 10965, USA., Phelan L; Pfizer Vaccine Research, 401 N. Middletown Road, Pearl River, NY 10965, USA., Green BA; Pfizer Vaccine Research, 401 N. Middletown Road, Pearl River, NY 10965, USA., Jansen KU; Pfizer Vaccine Research, 401 N. Middletown Road, Pearl River, NY 10965, USA., Pavliak V; Pfizer Vaccine Research, 401 N. Middletown Road, Pearl River, NY 10965, USA.
Jazyk: angličtina
Zdroj: Carbohydrate research [Carbohydr Res] 2014 Jan 13; Vol. 383, pp. 97-104. Date of Electronic Publication: 2013 Nov 19.
DOI: 10.1016/j.carres.2013.11.006
Abstrakt: We report herein the previously unknown structures of the pneumococcal capsular polysaccharides serotype 33C and 33D, and a revised structure of serotype 33B. The syntenic pair 33B/33D has nearly identical polysaccharide repeat units with the exception of one sugar residue (→2-α-Glcp in 33B and →2-α-Galp in 33D). Serotype 33C is structurally more similar to 33B/33D than 33A/33F, in that it also possesses a backbone ribitol-phosphate group and a →3-β-GalpNAc residue, both of which are absent in the repeat units of 33A/33F. Serotype 33C is notably different from all other serogroup 33 polysaccharides, as there is no →3-β-Glcp residue and the location of the O-acetylation of the →5-β-Galf residue (O-6) differs from the other serogroup 33 polysaccharides (O-2). This completes the structural assignments of polysaccharides within serogroup 33 and provides a framework for understanding the recognition of epitopes by serogroup 33 typing sera based on observed cross-reactivities reported in the literature.
(Copyright © 2013 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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