Genome-wide mapping of DNA methylation in the human malaria parasite Plasmodium falciparum.

Autor:	Ponts N; Department of Cell Biology and Neuroscience, University of California, 900 University Avenue, Riverside, CA 92521, USA; INRA, UR1264-MycSA, 71 Avenue E. Bourlaux, CS20032, 33882 Villenave d'Ornon Cedex, France., Fu L; Department of Chemistry, University of California, 900 University Avenue, Riverside, CA 92521, USA., Harris EY; Department of Computer Science and Engineering, University of California, 900 University Avenue, Riverside, CA 92521, USA., Zhang J; Department of Chemistry, University of California, 900 University Avenue, Riverside, CA 92521, USA; School of Chemistry & Materials Science, Shaanxi Normal University, 199 South Chang'an Road, Xi'an 710062, China., Chung DW; Department of Cell Biology and Neuroscience, University of California, 900 University Avenue, Riverside, CA 92521, USA., Cervantes MC; Department of Cell Biology and Neuroscience, University of California, 900 University Avenue, Riverside, CA 92521, USA., Prudhomme J; Department of Cell Biology and Neuroscience, University of California, 900 University Avenue, Riverside, CA 92521, USA., Atanasova-Penichon V; INRA, UR1264-MycSA, 71 Avenue E. Bourlaux, CS20032, 33882 Villenave d'Ornon Cedex, France., Zehraoui E; INRA, UR1264-MycSA, 71 Avenue E. Bourlaux, CS20032, 33882 Villenave d'Ornon Cedex, France., Bunnik EM; Department of Cell Biology and Neuroscience, University of California, 900 University Avenue, Riverside, CA 92521, USA., Rodrigues EM; Department of Cell Biology and Neuroscience, University of California, 900 University Avenue, Riverside, CA 92521, USA., Lonardi S; Department of Computer Science and Engineering, University of California, 900 University Avenue, Riverside, CA 92521, USA., Hicks GR; Center for Plant Cell Biology and Department of Botany & Plant Sciences, University of California, 900 University Avenue, Riverside, CA 92521, USA., Wang Y; Department of Chemistry, University of California, 900 University Avenue, Riverside, CA 92521, USA., Le Roch KG; Department of Cell Biology and Neuroscience, University of California, 900 University Avenue, Riverside, CA 92521, USA. Electronic address: karine.leroch@ucr.edu.
Jazyk:	angličtina
Zdroj:	Cell host & microbe [Cell Host Microbe] 2013 Dec 11; Vol. 14 (6), pp. 696-706.
DOI:	10.1016/j.chom.2013.11.007
Abstrakt:	Cytosine DNA methylation is an epigenetic mark in most eukaryotic cells that regulates numerous processes, including gene expression and stress responses. We performed a genome-wide analysis of DNA methylation in the human malaria parasite Plasmodium falciparum. We mapped the positions of methylated cytosines and identified a single functional DNA methyltransferase (Plasmodium falciparum DNA methyltransferase; PfDNMT) that may mediate these genomic modifications. These analyses revealed that the malaria genome is asymmetrically methylated and shares common features with undifferentiated plant and mammalian cells. Notably, core promoters are hypomethylated, and transcript levels correlate with intraexonic methylation. Additionally, there are sharp methylation transitions at nucleosome and exon-intron boundaries. These data suggest that DNA methylation could regulate virulence gene expression and transcription elongation. Furthermore, the broad range of action of DNA methylation and the uniqueness of PfDNMT suggest that the methylation pathway is a potential target for antimalarial strategies. (Copyright © 2013 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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