Procedure for microbial identification based on Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry from screening-positive urine samples.

Autor: March Rosselló GA; Service of Microbiology, University Clinic Hospital of Valladolid, Valladolid, Spain., Gutiérrez Rodríguez MP, de Lejarazu Leonardo RO, Orduña Domingo A, Bratos Pérez MA
Jazyk: angličtina
Zdroj: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [APMIS] 2014 Sep; Vol. 122 (9), pp. 790-5. Date of Electronic Publication: 2013 Dec 10.
DOI: 10.1111/apm.12208
Abstrakt: Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry is a widely used proteomic technique in clinical microbiology laboratories, and enables microbial identification directly from clinical samples. This study seeks to establish a protocol for bacterial identification from monomicrobial urine samples that have tested positive in the screening with Sysmex UF-1000i (Sysmex Corporation, Kobe, Japan). Sysmex UF-1000i counts ≥1 × 10(7) bacteria/mL indicate a sufficient bacterial concentration to allow direct identification from urine, with 87.5% sensitivity. Microbial identification from urine with Sysmex UF-1000i counts between 1 × 10(5) and 1 × 10(7) bacteria/ml requires preincubation to obtain the adequate amount of bacteria needed for analysis, and 91.7% sensitivity thus being achieved.
(© 2013 APMIS. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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