| Autor: |
Dagda RK; Pharmacology Department; University of Nevada, Reno; Reno, NV 89557; ; Biology Department, Community College of Allegheny County, Pittsburgh, PA, 15122, US;, Thalhauser RM, Dagda R, Marzullo TC, Gage GJ |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience [J Undergrad Neurosci Educ] 2013 Oct 15; Vol. 12 (1), pp. A66-74. Date of Electronic Publication: 2013 Oct 15 (Print Publication: 2013). |
| Abstrakt: |
Anatomy and physiology instructors often face the daunting task of teaching the principles of neurophysiology as part of a laboratory course with very limited resources. Teaching neurophysiology can be a difficult undertaking as sophisticated electrophysiology and data acquisition equipment is often financially out-of-reach for two-year institutions, and for many preparations, instructors need to be highly skilled in electrophysiology techniques when teaching hands-on laboratories. In the absence of appropriate laboratory tools, many undergraduate students have difficulty understanding concepts related to neurophysiology. The cricket can serve as a reliable invertebrate model to teach the basic concepts of neurophysiology in the educational laboratory. In this manuscript, we describe a series of hands-on, demonstrative, technologically simple, and affordable laboratory activities that will help undergraduate students gain an understanding of the principles of neurophysiology. By using the cerci ganglion and leg preparation, students can quantify extracellular neural activity in response to sensory stimulation, understand the principles of rate coding and somatotopy, perform electrical microstimulation to understand the threshold of sensory stimulation, and do pharmacological manipulation of neuronal activity. We describe the utility of these laboratory activities, provide a convenient protocol for quantifying extracellular recordings, and discuss feedback provided by undergraduate students with regards to the quality of the educational experience after performing the lab activities. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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