Measurement of downstream kinase activity modulation as indicator of epidermal growth factor receptor inhibitor efficacy.
Autor: | Dickerson WM; BioScale, Inc., 4 Maguire Road, Lexington, MA 02421, USA. Electronic address: mdickerson@bioscale.com., Saab A; BioScale, Inc., 4 Maguire Road, Lexington, MA 02421, USA., Leong K; BioScale, Inc., 4 Maguire Road, Lexington, MA 02421, USA., Miller M; BioScale, Inc., 4 Maguire Road, Lexington, MA 02421, USA., Latterich M; BioScale, Inc., 4 Maguire Road, Lexington, MA 02421, USA., Beausang LA; BioScale, Inc., 4 Maguire Road, Lexington, MA 02421, USA., Alderman EM; BioScale, Inc., 4 Maguire Road, Lexington, MA 02421, USA; Immunologic Consulting, 20 Cahill Park Drive, Framingham, MA 01702, USA. |
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Jazyk: | angličtina |
Zdroj: | Analytical biochemistry [Anal Biochem] 2014 Mar 01; Vol. 448, pp. 65-7. Date of Electronic Publication: 2013 Dec 06. |
DOI: | 10.1016/j.ab.2013.11.032 |
Abstrakt: | The acoustic membrane micro particle (AMMP) technology has been used to quantify single analytes out of multiple sample types. In this study the technology is used to reveal molecular interactions of components of kinase pathways. Specifically, the downstream kinase activity of the EGFR receptor in the presence or absence of EGFR inhibitors is investigated. These experiments substantiate that EGFR stimulation predominantly activates the MEK/ERK pathway. The EGFR inhibitors tested had varying effectiveness at preventing phosphorylation at the EGFR or downstream kinase activity. These experiments reveal the use of the AMMP technology for observing multiple signaling pathways in a single experiment. (Copyright © 2013 Elsevier Inc. All rights reserved.) |
Databáze: | MEDLINE |
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