Phylogenetic analysis of the polyprotein coding region of an infectious South African bursal disease virus (IBDV) strain.

Autor: Vukea PR; School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, South Africa., Willows-Munro S; School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, South Africa., Horner RF; Vetdiagnostix, Veterinary Pathology Services, Pietermaritzburg, KwaZulu-Natal, South Africa., Coetzer TH; School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, South Africa. Electronic address: Coetzer@ukzn.ac.za.
Jazyk: angličtina
Zdroj: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2014 Jan; Vol. 21, pp. 279-86. Date of Electronic Publication: 2013 Dec 01.
DOI: 10.1016/j.meegid.2013.11.017
Abstrakt: Infectious bursal disease virus (IBDV) causes Gumboro disease, which is highly contagious and immunosuppressive in young chickens. A virulent form of IBDV reached South Africa in 1989 and to date there has been little molecular information available for this strain. In this study, the polyprotein coding region of the South African strain SA-KZN95 was sequenced and analysed along with 52 representative sequences of other serotype I and II strains. We explored the relative impact of recombination on phylogenetic reconstruction using a multidimensional scaling approach. Phylogenetic analyses consistently placed the South African isolate within the very virulent IBDV clade. Selection analyses were also conducted to identify evolutionarily relevant amino acid residues. Previously, 19 residues in the polyprotein were shown to be potentially diagnostic for the different IBDV pathotypes. This study identified an additional two unique residues in the polyprotein which may be used as genetic signatures in future viral identifications. Better strain identification would aid in the development and application of vaccines.
(Copyright © 2013 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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