Superoxide dismutase-loaded biodegradable nanoparticles targeted with a follicle-stimulating hormone peptide protect Sertoli cells from oxidative stress.

Autor:	Snow-Lisy DC; Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio; Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio. Electronic address: snowlid@ccf.org., Sabanegh ES Jr; Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio., Samplaski MK; Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio., Morris VB; Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio., Labhasetwar V; Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio.
Jazyk:	angličtina
Zdroj:	Fertility and sterility [Fertil Steril] 2014 Feb; Vol. 101 (2), pp. 560-7. Date of Electronic Publication: 2013 Nov 26.
DOI:	10.1016/j.fertnstert.2013.10.034
Abstrakt:	Objective: To evaluate targeted superoxide dismutase (SOD)-loaded biodegradable nanoparticles' (NPs) ability to protect Sertoli cells from hydrogen peroxide (H2O2)-induced oxidative stress. Design: Cell culture controlled experimental study. Setting: Research laboratory. Cells: Mouse testis Sertoli cells (TM4). Interventions: Sertoli cells were exposed to 0-200 μg/mL plain media, unconjugated NPs, or FSH peptide-conjugated NPs for 2 or 24 hours to assess uptake. Next, Sertoli cells were exposed to 0-50 mmol H₂O₂ with 0-1 mg/mL unconjugated SOD-loaded NPs, FSH-conjugated SOD-loaded NPs, or equivalent units of SOD in solution as a control for 2-6 hours to assess influence on cell survival after oxidative stress. Main Outcome Measure(s): Cell viability, flow cytometry, and microscopy. Result(s): FSH peptide targeting improved uptake of NPs by Sertoli cells. FSH-conjugated SOD-NPs significantly protected Sertoli cells at 6 hours of H₂O₂--induced oxidative stress, with 100% survival with FSH-conjugated SOD-NPs compared with unconjugated SOD-NPs (45%) or SOD in solution (36%). Conclusion(s): Conjugation of NPs with FSH peptide improves cellular uptake and survival when SOD-loaded NPs are coincubated with Sertoli cells undergoing oxidative stress. This study represents a step toward developing NPs for the targeted treatment of testicular oxidative stress. (Copyright © 2014 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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