An in vitro study on the photosensitivity of 5-aminolevulinic acid conjugated gold nanoparticles.

Autor: Mohammadi Z; Department and Research Center of Medical Physics, Babol University of Medical Sciences, Babol, Iran. Electronic address: Mehr_055@yahoo.com., Sazgarnia A, Rajabi O, Soudmand S, Esmaily H, Sadeghi HR
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2013 Dec; Vol. 10 (4), pp. 382-8. Date of Electronic Publication: 2013 May 04.
DOI: 10.1016/j.pdpdt.2013.03.010
Abstrakt: Background: In this study, the photosensitivity of 5-aminolevulinic acid (5-ALA) conjugated gold nanoparticles (conjugate) has been investigated on the MeL-Rm cell line.
Methods: After culture and proliferation of the Mel-Rm cell line, cell toxicity and the suitable cell incubation time of the conjugate were determined. Photosensitivity of the conjugate and free 5-ALA was then studied in optical doses of 20, 40, 60 and 80 J/cm(2) while light irradiation was applied by a He-Ne laser.
Results: A 4-h time interval was determined as the optimum incubation time for 5-ALA and the conjugate. PpIX induction into the cells showed a significant increase after incubation with the conjugate in comparison to 5-ALA alone. Also, the conjugate resulted in a two times higher cell death rate compared to free 5-ALA.
Conclusion: The conjugate can be used as an appropriate delivery agent for 5-ALA and provide efficient cell destruction during PDT.
(Copyright © 2013 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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