| Autor: |
Easterling DE; Bioavailability/Pharmacokinetics Section, Department of Drug Metabolism, McNeil Pharmaceutical, Spring House, Pennsylvania, 19477., de Torres WR, Desiraju RK |
| Jazyk: |
angličtina |
| Zdroj: |
Pharmaceutical research [Pharm Res] 1986 Feb; Vol. 3 (1), pp. 45-7. |
| DOI: |
10.1023/A:1016372815539 |
| Abstrakt: |
An automated, sensitive, and selective reverse-phase high-performance liquid chromatographic assay has been developed to measure codeine in plasma. The analysis requires only 1 ml plasma and is accomplished by detection of the fluorescence of codeine following extraction and concentration. The method is simple and rapid, involving a one-step extraction of codeine from alkalinized (pH 10.0) plasma into an organic layer of hexane/dichloromethane, 2/1. The organic layer was evaporated under nitrogen and the residue reconstituted with the mobile phase. The samples were chromatographed on a reverse-phase C-18 column using a mobile phase of acetonitrile-phosphate buffer, 80/20 (pH 5.80). The codeine and internal standard, N-allylnorcodeine, peaks were detected using a fluorescence detector. The retention times were 8.6 min for the internal standard and 11.3 min for codeine. Standard curves were linear from 10 to 250 ng/ml. The assay was validated by direct comparison with a gas chromatographic procedure that employed nitrogen-phosphorus detection. The assay has been employed for the analysis of several codeine studies using human, dog, and rat plasma. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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