[Characterization of epithelial primary culture from human conjunctiva].

Autor:	Rivas L; Servicio de Oftalmología, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, España. Electronic address: lrivas53@gmail.com., Blázquez A; Laboratorio de Ingeniería Celular, Hospital Universitario La Paz, Madrid, España., Muñoz-Negrete FJ; Servicio de Oftalmología, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, España., López S; Servicio de Oftalmología, Hospital Central de la Cruz Roja, Madrid, España., Rebolleda G; Servicio de Oftalmología, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, España., Domínguez F; Laboratorio de Ingeniería Celular, Hospital Universitario La Paz, Madrid, España., Pérez-Esteban A; Servicio de Oftalmología, Hospital Universitario La Paz, Madrid, España.
Jazyk:	Spanish; Castilian
Zdroj:	Archivos de la Sociedad Espanola de Oftalmologia [Arch Soc Esp Oftalmol] 2014 Jan; Vol. 89 (1), pp. 10-6. Date of Electronic Publication: 2013 Oct 05.
DOI:	10.1016/j.oftal.2013.07.013
Abstrakt:	Objective: To evaluate primary cultures from human conjunctiva supplemented with fetal bovine serum, autologous serum, and platelet-rich autologous serum, over human amniotic membrane and lens anterior capsules. Methods: One-hundred and forty-eight human conjunctiva explants were cultured in CnT50(®) supplemented with 1, 2.5, 5 and 10% fetal bovine serum, autologous serum and platelet-rich autologous serum. Conjunctival samples were incubated at 37°C, 5% CO2 and 95% HR, for 3 weeks. Results: The typical phenotype corresponding to conjunctival epithelial cells was present in all primary cultures. Conjunctival cultures had MUC5AC-positive secretory cells, K19-positive conjunctival cells, and MUC4-positive non-secretory conjunctival cells, but were not corneal phenotype (cytokeratin K3-negative) and fibroblasts (CD90-negative). Conclusions: Conjunctiva epithelial progenitor cells were preserved in all cultures; thus, a cell culture in CnT50(®) supplemented with 1 to 5% autologous serum over human amniotic membrane can provide better information of epithelial cell differentiation for the conjunctival surface reconstruction. (Copyright © 2013 Sociedad Española de Oftalmología. Published by Elsevier Espana. All rights reserved.)
Databáze:	MEDLINE
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