| Autor: |
Macente S; Post Graduation Program in Biosciences Applied to Pharmacy, Department of Clinical Analyses and Biomedicine, State University of Maringa, 87020-900 Maringá, PR, Brazil., Fujimura Leite CQ, Santos AC, Siqueira VL, Machado LN, Marcondes NR, Hirata MH, Hirata RD, Cardoso RF |
| Jazyk: |
angličtina |
| Zdroj: |
BioMed research international [Biomed Res Int] 2013; Vol. 2013, pp. 391549. Date of Electronic Publication: 2013 Oct 24. |
| DOI: |
10.1155/2013/391549 |
| Abstrakt: |
Current study evaluated the hsp65 Nested PCR Restriction Fragment Length Polymorphism Analysis (hsp65 Nested PCR-PRA) to detect and identify Mycobacterium tuberculosis complex directly in clinical samples for a rapid and specific diagnosis of tuberculosis (TB). hsp65 Nested PCR-PRA was applied directly to 218 clinical samples obtained from 127 patients suspected of TB or another mycobacterial infection from July 2009 to July 2010. The hsp65 Nested PCR-PRA showed 100% sensitivity and 95.0 and 93.1% specificity in comparison with culture and microscopy (acid fast bacillus smear), respectively. hsp65 Nested PCR-PRA was shown to be a fast and reliable assay for diagnosing TB, which may contribute towards a fast diagnosis that could help the selection of appropriate chemotherapeutic and early epidemiological management of the cases which are of paramount importance in a high TB burden country. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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