Polysaccharides from Ganoderma formosanum function as a Th1 adjuvant and stimulate cytotoxic T cell response in vivo.

Autor: Pi CC; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Chu CL; Graduate Institute of Immunology, National Taiwan University, College of Medicine, Taipei 100, Taiwan, ROC., Lu CY; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Zhuang YJ; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Wang CL; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Yu YH; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Wang HY; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Lin CC; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC., Chen CJ; Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC. Electronic address: cjchen1@ntu.edu.tw.
Jazyk: angličtina
Zdroj: Vaccine [Vaccine] 2014 Jan 09; Vol. 32 (3), pp. 401-8. Date of Electronic Publication: 2013 Nov 17.
DOI: 10.1016/j.vaccine.2013.11.027
Abstrakt: The fungus of Ganoderma is a basidiomycete that possesses a variety of pharmacological effects and has been used in traditional Asian medicine for centuries. Ganoderma formosanum is a native Ganoderma species isolated in Taiwan, and we have previously demonstrated that PS-F2, a polysaccharide fraction purified from the submerged culture broth of G. formosanum, exhibits immunostimulatory properties in macrophages. In this study, we further characterized the adjuvant functions of PS-F2. In vitro, PS-F2 stimulated dendritic cells (DCs) to produce proinflammatory cytokines, including TNF-α, interleukin (IL)-6, and IL-12/IL-23 p40. PS-F2 also stimulated DCs to express the maturation markers CD40, CD80, CD86, and MHC class II. In a murine splenocyte culture, PS-F2 treatment resulted in elevated expression of T-bet and interferon (IFN)-γ in T lymphocytes. When used as an adjuvant in vivo with the ovalbumin (OVA) antigen, PS-F2 stimulated OVA-specific antibody production and primed IFN-γ production in OVA-specific T lymphocytes. PS-F2-adjuvated immunization also induced OVA-specific CTLs, which protected mice from a challenge with tumor cells expressing OVA. Collectively, our data show that PS-F2 functions as an adjuvant capable of inducing a Th1-polarized adaptive immune response, which would be useful in vaccines against viruses and tumors.
(Copyright © 2013 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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