| Autor: |
Fry J; Biological Sciences, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA., Barnason A, Horsch RB |
| Jazyk: |
angličtina |
| Zdroj: |
Plant cell reports [Plant Cell Rep] 1987 Oct; Vol. 6 (5), pp. 321-5. |
| DOI: |
10.1007/BF00269550 |
| Abstrakt: |
A reproducible system to produce transgenic Brassica napus plants has been developed using stem segments. Stem segments from 6-7 week old plants were inoculated with an Agrobacterium tumefaciens strain containing a disarmed tumor-inducing plasmid pTiT37-SE carrying a chimeric bacterial gene encoding kanamycin resistance (pMON200). Stem explants were cocultured for 2 days before transfer to kanamycin selection medium. Shoots regenerated directly from the explant in 3-6 weeks and were excised, dipped in Rootone®, and rooted in soil. Transformation was confirmed by opine production, kanamycin resistance, and DNA blot hybridization in the primary transformants. Final proof of transformation was demonstrated by the co-transfer of opine production and kanamycin resistance to progeny in a Mendelian fashion. Over 200 transgenic Brassica napus plants have been produced using this system. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink