Histologic characterization of engineered tissues in the canal space of closed-apex teeth with apical periodontitis.
| Autor: | Gomes-Filho JE; Department of Endodontics, Araçatuba School of Dentistry, Universidade Estadual Paulista, Araçatuba, São Paulo, Brazil. Electronic address: jegomesfilho@yahoo.com.br., Duarte PC, Ervolino E, Mogami Bomfim SR, Xavier Abimussi CJ, Mota da Silva Santos L, Lodi CS, Penha De Oliveira SH, Dezan E Jr, Cintra LT |
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| Jazyk: | angličtina |
| Zdroj: | Journal of endodontics [J Endod] 2013 Dec; Vol. 39 (12), pp. 1549-56. Date of Electronic Publication: 2013 Sep 29. |
| DOI: | 10.1016/j.joen.2013.08.023 |
| Abstrakt: | Introduction: The aim of this study was to investigate the capacity of endodontic regenerative procedures combining an induced blood clot, platelet-rich plasma (PRP), and bone marrow aspirate (BMA) to regenerate dental pulp in canine closed-apex necrotic teeth. Methods: Apical periodontitis was induced in 20 upper and lower premolars of 2 dogs. After biomechanical preparation, enlargement to a #60 file, and disinfection with a triantibiotic paste for 28 days, the roots were randomly assigned to 4 treatment groups: blood clot (BC), BC + PRP gel, BC + BMA gel, and BC + BMA/PRP gel. Negative controls were also included. After a 3-month follow-up period, the animals were killed. Results: Histologic analysis showed the presence of newly formed vital tissues (connective, cement-like, and bone-like tissue) in 23 of the 32 treated roots (71.87%). There was no statistically significant difference between the treatment groups. Conclusions: New vital tissues were formed and characterized as connective, cementum-like, or bone-like, but not as pulp-like tissue; PRP and/or BMA did not improve the tissue ingrowth. (Copyright © 2013 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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