| Autor: |
Kotova ES; Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. akser@ibch.ru., Sorokina IV, Akopov SB, Nikolaev LG, Sverdlov ED |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2013 Aug; Vol. 78 (8), pp. 879-83. |
| DOI: |
10.1134/S0006297913080038 |
| Abstrakt: |
The chicken gene for transcription factor CTCF was expressed in COS-1 mammalian cells. The CTCF protein containing polyhistidine tag was partially purified using metallo-affinity and ion-exchange chromatography. The expressed protein localized in the cell nucleus and was shown to be functionally active in the electrophoretic mobility shift assay and specifically interacted with anti-CTCF antibodies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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