Detection of viral and bacterial pathogens in acute respiratory infections.

Autor: Obasi CN; Department of Family Medicine, University of Wisconsin-Madison, School of Medicine and Public Health, 1100 Delaplaine Ct., Madison, WI 53715, USA. Electronic address: cobasi@wisc.edu., Barrett B; Department of Family Medicine, University of Wisconsin-Madison, School of Medicine and Public Health, 1100 Delaplaine Ct., Madison, WI 53715, USA., Brown R; Schools of Nursing, Medicine and Public Health, Research Design & Statistics Unit, University of Wisconsin-Madison, USA., Vrtis R; School of Medicine, Departments of Pediatrics and Medicine, University of Wisconsin-Madison, USA., Barlow S; Department of Family Medicine, University of Wisconsin-Madison, School of Medicine and Public Health, 1100 Delaplaine Ct., Madison, WI 53715, USA., Muller D; Department of Medicine - Rheumatology, University of Wisconsin-Madison, School of Medicine and Public Health, USA., Gern J; School of Medicine, Departments of Pediatrics and Medicine, University of Wisconsin-Madison, USA.
Jazyk: angličtina
Zdroj: The Journal of infection [J Infect] 2014 Feb; Vol. 68 (2), pp. 125-30. Date of Electronic Publication: 2013 Nov 07.
DOI: 10.1016/j.jinf.2013.10.013
Abstrakt: Objectives: The role of bacteria in acute respiratory illnesses (ARI) of adults and interactions with viral infections is incompletely understood. This study tested the hypothesis that bacterial co-infection during ARI adds to airway inflammation and illness severity.
Methods: Two groups of 97 specimens each were randomly selected from multiplex-PCR identified virus-positive and virus-negative nasal specimens obtained from adults with new onset ARI, and 40 control specimens were collected from healthy adults. All specimens were analyzed for Haemophilus influenzae(HI), Moraxella catarrhalis(MC) and Streptococcus pneumoniae(SP) by quantitative-PCR. General linear models tested for relationships between respiratory pathogens, biomarkers (nasal wash neutrophils and CXCL8), and ARI-severity.
Results: Nasal specimens from adults with ARIs were more likely to contain bacteria (37% overall; HI = 28%, MC = 14%, SP = 7%) compared to specimens from healthy adults (5% overall; HI = 0%, MC = 2.5%, SP = 2.5%; p < 0.001). Among ARI specimens, bacteria were more likely to be detected among virus-negative specimens compared to virus-positive specimens (46% vs. 27%; p = 0.0046). The presence of bacteria was significantly associated with increased CXCL8 and neutrophils, but not increased symptoms.
Conclusion: Pathogenic bacteria were more often detected in virus-negative ARI, and also associated with increased inflammatory biomarkers. These findings suggest the possibility that bacteria may augment virus-induced ARI and contribute to airway inflammation.
(Copyright © 2013 The British Infection Association. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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