| Autor: |
Newton RJ; Department of Forest Science, Texas Agricultural Experiment Station, Texas A & M University System, 77843-2135, College Station, TX., Yibrah HS, Dong N, Clapham DH, von Arnold S |
| Jazyk: |
angličtina |
| Zdroj: |
Plant cell reports [Plant Cell Rep] 1992 May; Vol. 11 (4), pp. 188-91. |
| DOI: |
10.1007/BF00232530 |
| Abstrakt: |
The 1.5 kilobase promoter sequence upstream of Dc8, a late embryo abundant gene of Daucus, fused to the reporter β-glucuronidase gene was introduced into several tissues of Picea abies via a custom-made electric-discharge particle accelerator. Transient expression was measured histochemically as spot number 2 d after bombardment. Embryogenic suspensions gave higher levels of expression depending upon cell line than embryogenic callus or zygotic embryos. Expression was enhanced when cultures were treated with abscisic acid for 3 d before bombardment. A mean and maximum of 17 and 34 spots/disk, respectively, were observed with the best cell line, which was comparable with the level of expression driven by an enhanced 35S promoter. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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