[Relationship between the expressions of indoleamine 2, 3-dioxygenase in hepatocellular carcinoma and clinicopathological parameters].

Autor:	Lin L; Department of Hepatobiliary Surgery & Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China., Yang DH, Huang Y, Li XH, Zhang QL, Liu X, Liang JK, Zhong KB, He H
Jazyk:	čínština
Zdroj:	Zhonghua yi xue za zhi [Zhonghua Yi Xue Za Zhi] 2013 Jul 23; Vol. 93 (28), pp. 2186-90.
Abstrakt:	Objective: To explore the expressions of indoleamine 2, 3-dioxygenase (IDO) in hepatocellular carcinoma and analyze its relationship with clinicopathological parameters. Methods: Quantitative real-time polymerase chain reaction (PCR), fluorogenic quantitative PCR, immunohistochemical and immunofluorescence were used to detect the expression of indoleamine 2, 3-dioxygenase in hepatocellular carcinoma. Results: The IDO mRNA expression in cancerous tissues increased markedly than that in the corresponding non-cancerous tissues (2(-ΔΔCT) = 1.71, P = 0.001) . The immunohistochemical and immunofluorescence results showed that IDO protein was expressed in cytoplasm of hepatocellular carcinoma and tumor-surrounding tissues. But there was no expression in normal liver tissues from benign hepatic lesions and corresponding non-cancerous tissues. An over-expression of IDO protein was detected in 43 patients (48.3%) , a low expression in 25 (28.1%) and no expression in 21 (23.6%). Relationship between IDO expression and clinicopathological parameters: an over-expression of IDO in HCC was associated with recurrence, survival time, metastasis and TNM stage (P < 0.05), but not associated with patient's cirrhosis, AFP level, histological differentiation type, Barcelona clinic liver cancer stage, gender, age, HbsAg positivity, number of tumors and tumor size (P > 0.05). Conclusion: An over-expression of IDO in HCC patients may affect patient prognosis.
Databáze:	MEDLINE
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