| Autor: |
Cheng CS; Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA., Rai K, Garber M, Hollinger A, Robbins D, Anderson S, Macbeth A, Tzou A, Carneiro MO, Raychowdhury R, Russ C, Hacohen N, Gershenwald JE, Lennon N, Nusbaum C, Chin L, Regev A, Amit I |
| Jazyk: |
angličtina |
| Zdroj: |
Nature communications [Nat Commun] 2013; Vol. 4, pp. 2672. |
| DOI: |
10.1038/ncomms3672 |
| Abstrakt: |
The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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