Production of medium chain length fatty alcohols from glucose in Escherichia coli.

Autor: Youngquist JT; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 3629 Engineering Hall, 1415 Engineering Drive, Madison, WI 53706, United States; Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI 53706, United States., Schumacher MH, Rose JP, Raines TC, Politz MC, Copeland MF, Pfleger BF
Jazyk: angličtina
Zdroj: Metabolic engineering [Metab Eng] 2013 Nov; Vol. 20, pp. 177-86. Date of Electronic Publication: 2013 Oct 17.
DOI: 10.1016/j.ymben.2013.10.006
Abstrakt: Metabolic engineering offers the opportunity to produce a wide range of commodity chemicals that are currently derived from petroleum or other non-renewable resources. Microbial synthesis of fatty alcohols is an attractive process because it can control the distribution of chain lengths and utilize low cost fermentation substrates. Specifically, primary alcohols with chain lengths of 12 to 14 carbons have many uses in the production of detergents, surfactants, and personal care products. The current challenge is to produce these compounds at titers and yields that would make them economically competitive. Here, we demonstrate a metabolic engineering strategy for producing fatty alcohols from glucose. To produce a high level of 1-dodecanol and 1-tetradecanol, an acyl-ACP thioesterase (BTE), an acyl-CoA ligase (FadD), and an acyl-CoA/aldehyde reductase (MAACR) were overexpressed in an engineered strain of Escherichia coli. Yields were improved by balancing expression levels of each gene, using a fed-batch cultivation strategy, and adding a solvent to the culture for extracting the product from cells. Using these strategies, a titer of over 1.6 g/L fatty alcohol with a yield of over 0.13 g fatty alcohol/g carbon source was achieved. These are the highest reported yield of fatty alcohols produced from glucose in E. coli.
(© 2013 Published by Elsevier Inc.)
Databáze: MEDLINE
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