| Autor: |
Pfeilsticker JA; Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California, United States of America., Umeda A, Farrow B, Hsueh CL, Deyle KM, Kim JT, Lai BT, Heath JR |
| Jazyk: |
angličtina |
| Zdroj: |
PloS one [PLoS One] 2013 Oct 07; Vol. 8 (10), pp. e76224. Date of Electronic Publication: 2013 Oct 07 (Print Publication: 2013). |
| DOI: |
10.1371/journal.pone.0076224 |
| Abstrakt: |
We report on a method to improve in vitro diagnostic assays that detect immune response, with specific application to HIV-1. The inherent polyclonal diversity of the humoral immune response was addressed by using sequential in situ click chemistry to develop a cocktail of peptide-based capture agents, the components of which were raised against different, representative anti-HIV antibodies that bind to a conserved epitope of the HIV-1 envelope protein gp41. The cocktail was used to detect anti-HIV-1 antibodies from a panel of sera collected from HIV-positive patients, with improved signal-to-noise ratio relative to the gold standard commercial recombinant protein antigen. The capture agents were stable when stored as a powder for two months at temperatures close to 60(o)C. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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