| Autor: |
Kolodkin-Gal D; Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA., Hulot SL, Korioth-Schmitz B, Gombos RB, Zheng Y, Owuor J, Lifton MA, Ayeni C, Najarian RM, Yeh WW, Asmal M, Zamir G, Letvin NL |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of virology [J Virol] 2013 Dec; Vol. 87 (24), pp. 13589-97. Date of Electronic Publication: 2013 Oct 09. |
| DOI: |
10.1128/JVI.03108-12 |
| Abstrakt: |
Effective strategies are needed to block mucosal transmission of human immunodeficiency virus type 1 (HIV-1). Here, we address a crucial question in HIV-1 pathogenesis: whether infected donor mononuclear cells or cell-free virus plays the more important role in initiating mucosal infection by HIV-1. This distinction is critical, as effective strategies for blocking cell-free and cell-associated virus transmission may be different. We describe a novel ex vivo model system that utilizes sealed human colonic mucosa explants and demonstrate in both the ex vivo model and in vivo using the rectal challenge model in rhesus monkeys that HIV-1-infected lymphocytes can transmit infection across the mucosa more efficiently than cell-free virus. These findings may have significant implications for our understanding of the pathogenesis of mucosal transmission of HIV-1 and for the development of strategies to prevent HIV-1 transmission. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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