| Autor: |
Schneider T; Department of Chemistry, University of Washington , Seattle, Washington 98195-1700, United States., Yen GS, Thompson AM, Burnham DR, Chiu DT |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical chemistry [Anal Chem] 2013 Nov 05; Vol. 85 (21), pp. 10417-23. Date of Electronic Publication: 2013 Oct 07. |
| DOI: |
10.1021/ac402383n |
| Abstrakt: |
This paper describes a sample digitization method that generates tens of thousands of nanoliter-sized droplets in a high-density array in a matter of minutes. We show that the sample digitization depends on both the geometric design of the microfluidic device and the viscoelastic forces between the aqueous sample and a continuous oil phase. Our design avoids sample loss: Samples are split into tens of thousands of discrete volumes with close to 100% efficiency without the need for any expensive valving or pumping systems. We envision this technology will have broad applications that require simple sample digitization within minutes, such as digital polymerase chain reactions and single-cell studies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
