Production of asiaticoside from centella (Centella asiatica L. Urban) cells in bioreactor.
| Autor: | Loc NH; Institute of Resources, Environment and Biotechnology, Hue University, Hue 47000, Vietnam. Electronic address: nhloc@hueuni.edu.vn., Nhat NT |
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| Jazyk: | angličtina |
| Zdroj: | Asian Pacific journal of tropical biomedicine [Asian Pac J Trop Biomed] 2013 Oct; Vol. 3 (10), pp. 806-10. Date of Electronic Publication: 2013 Sep 04. |
| DOI: | 10.1016/S2221-1691(13)60159-8 |
| Abstrakt: | Objective: To investigate the effects of some culture conditions on production of asiaticoside from centella (Centella asiatica L. Urban) cells cultured in 5-L bioreactor. Methods: The centell cell suspension culture was conducted in 5-L bioreactor to investigate the growth and asiaticoside accumulation under various conditions. Asiaticoside content was determined by HPLC analysis. Results: The results showed that the cell growth and asiaticoside accumulation peaked after 24 d of culture at an agitation speed of 150 r/min and aeration rate of 2.5 L/min. The cell biomass reached a maximum value of 302.45 g fresh weight (31.45 g dry weight) and growth index of 3.03 with inoculum size of 100 g. However, asiaticoside content was the highest (60.08 mg/g dry weight) when culture was initiated with an inoculum size of 50 g. Conclusions: The present study found the suitable conditions for growth of centella cells and their asiaticoside production in bioreactor. (Copyright © 2013 Asian Pacific Tropical Biomedical Magazine. Published by Elsevier B.V. All rights reserved.) |
| Databáze: | MEDLINE |
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