| Autor: |
Weiblen BJ; Massachusetts Department of Public Health, State Laboratory Institute, Jamaica Plain 02130., Schumacher RT, Hoff R |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of immunological methods [J Immunol Methods] 1990 Feb 09; Vol. 126 (2), pp. 199-204. |
| DOI: |
10.1016/0022-1759(90)90151-k |
| Abstrakt: |
Indirect assays for IgM and IgA antibodies often lack sensitivity and specificity due to interference from IgG antibodies. To overcome this problem we have developed a simple procedure using recombinant protein G coupled to agarose beads to remove the interfering IgG. A series of HIV seroconversion panels was tested by IgM and IgA immunoblot after protein G treatment in order to evaluate IgG removal and to study appearance of IgM and IgA antibodies in early HIV infection. Protein G treatment removed 99.9% of the IgG and reduced IgG anti-HIV titers of over 1/100,000 to undetectable levels. Both IgM and IgA HIV antibodies were detected as early in seroconversion as were IgG HIV antibodies. IgA HIV antibodies persisted for a longer period of time, reacted with more HIV proteins, and showed more intense staining than IgM HIV antibodies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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