| Autor: |
Kaplan MH; Departments of Pediatrics and Microbiology and Immunology; Indiana University School of Medicine; Indianapolis, IN USA., Cundiff JK, Smith JS, Aldrich CJ |
| Jazyk: |
angličtina |
| Zdroj: |
JAK-STAT [JAKSTAT] 2013 Apr 01; Vol. 2 (2), pp. e24554. |
| DOI: |
10.4161/jkst.24554 |
| Abstrakt: |
The generation of germline gene mutations in mice has been an invaluable tool for experimental biology. However, studying immune responses that develop in the absence of a specific protein that could alter thymic selection complicates experimental interpretations. We observed that CD8(+) T cells from Stat6 (-/-) mice displayed "autoreactivity" to STAT6-expressing cells, associated with specific STAT6 peptides binding to MHC class I molecules. These results suggest caution in interpreting experiments where STAT6-expressing cells are transferred into Stat6 (-/-) mice, or where adoptive transfer of Stat6 (-/-) lymphocytes is performed. Our results further highlight additional considerations when studying immune responses involving cell transfer into gene-deficient mice. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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