Nanoscale-targeted patch-clamp recordings of functional presynaptic ion channels.

Autor: Novak P; Department of Medicine, Imperial College London, London, W12 0NN, UK; School of Engineering and Materials Science, Queen Mary, University of London, London E1 4NS, UK. Electronic address: p.novak@qmul.ac.uk., Gorelik J, Vivekananda U, Shevchuk AI, Ermolyuk YS, Bailey RJ, Bushby AJ, Moss GW, Rusakov DA, Klenerman D, Kullmann DM, Volynski KE, Korchev YE
Jazyk: angličtina
Zdroj: Neuron [Neuron] 2013 Sep 18; Vol. 79 (6), pp. 1067-77.
DOI: 10.1016/j.neuron.2013.07.012
Abstrakt: Direct electrical access to presynaptic ion channels has hitherto been limited to large specialized terminals such as the calyx of Held or hippocampal mossy fiber bouton. The electrophysiology and ion-channel complement of far more abundant small synaptic terminals (≤ 1 μm) remain poorly understood. Here we report a method based on superresolution scanning ion conductance imaging of small synapses in culture at approximately 100-150 nm 3D resolution, which allows presynaptic patch-clamp recordings in all four configurations (cell-attached, inside-out, outside-out, and whole-cell). Using this technique, we report presynaptic recordings of K(+), Na(+), Cl(-), and Ca(2+) channels. This semiautomated approach allows direct investigation of the distribution and properties of presynaptic ion channels at small central synapses.
(Copyright © 2013 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE