| Autor: |
Quelch V; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences and College of Veterinary Medicine, University of Florida, Gainesville, 32611, USA., Reed KR, Erdos GW, Nicoletti PL, Hoffmann EM |
| Jazyk: |
angličtina |
| Zdroj: |
American journal of veterinary research [Am J Vet Res] 1984 Jul; Vol. 45 (7), pp. 1409-12. |
| Abstrakt: |
Phosphatidylcholine:cholesterol liposomes containing 4(5) carboxyfluorescein were modified by incorporation of Brucella abortus lipopolysaccharide. These vesicles were opsonized with bovine anti-B abortus or anti-B abortus plus complement. The fluorescent marker entrapped in antibody-opsonized liposomes was taken up by cultured bovine macrophages more effectively than was the same compound which had been loaded into nonopsonized liposomes. The addition of bovine complement to antibody-opsonized liposomes did not increase the extent of 4(5) carboxyfluorescein uptake over that caused by antibodies alone. The system, described in this report, could be used as a model for evaluation of the efficacy of selectively directing liposomes containing antibiotics to macrophages infected with intracellular B abortus, since macrophages possess Fc receptors capable of binding antibody-opsonized liposomes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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