Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: a possible role for SirT3 activation.

Autor: Teodoro JS; Center for Neuroscience and Cell Biology, Faculty of Science and Technology, University of Coimbra, 3004-517 Coimbra, Portugal; Department of Life Sciences, Faculty of Science and Technology, University of Coimbra, 3004-517 Coimbra, Portugal., Duarte FV, Gomes AP, Varela AT, Peixoto FM, Rolo AP, Palmeira CM
Jazyk: angličtina
Zdroj: Mitochondrion [Mitochondrion] 2013 Nov; Vol. 13 (6), pp. 637-46. Date of Electronic Publication: 2013 Sep 14.
DOI: 10.1016/j.mito.2013.09.002
Abstrakt: Berberine is an isoquinoline alkaloid with anti-diabetic properties. Despite the central role of liver and thus hepatic mitochondria in whole-body metabolism, berberine effects on hepatic mitochondrial function in an obesity model are still unknown. Here, we demonstrate that berberine treatment recovers mitochondrial efficiency when altered by a high-fat feeding. Mitochondria isolated from the liver of high-fat fed rats exhibited decreased capacity to accumulate calcium and impaired oxidative phosphorylation (OXPHOS) capacity, as shown by impaired mitochondrial membrane potential, oxygen consumption and cellular ATP levels. Interestingly, the recovery of mitochondrial function by berberine was associated with an increased activity of the mitochondrial sirtuin 3 (SirT3). In conclusion, berberine potent protective effects against metabolic syndrome may rely on increasing mitochondrial SirT3 activity, normalizing mitochondrial function and preventing a state of energetic deficit caused by impaired OXPHOS.
(© 2013.)
Databáze: MEDLINE
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