| Autor: |
Zamilpa R; Division of Geriatrics, Gerontology and Palliative Medicine, Department of Medicine, Barshop Institute of Longevity and Aging Studies, The University of Texas Health Science Center, San Antonio, TX, USA., Zhang J, Chiao YA, de Castro Brás LE, Halade GV, Ma Y, Hacker SO, Lindsey ML |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 1037, pp. 313-24. |
| DOI: |
10.1007/978-1-62703-505-7_18 |
| Abstrakt: |
Myocardial infarction (MI) is a leading cause of death worldwide. Permanent ligation of the left anterior descending coronary artery (LAD) is a commonly used surgical model to study post-MI effects in mice. LAD occlusion induces a robust wound healing response that includes extracellular matrix (ECM) remodeling. This chapter provides a detailed guide on the surgical procedure to permanently ligate the LAD. Additionally, we describe a prototype method to enrich cardiac tissue for ECM, which allows one to focus on ECM remodeling in the left ventricle following surgically induced MI in mice. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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