| Autor: |
Vu-Phan D; Cellular and Molecular Biology Graduate Program, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, 48109, USA., Grachtchouk V, Yu J, Colby LA, Wicha MS, Koenig RJ |
| Jazyk: |
angličtina |
| Zdroj: |
Endocrine-related cancer [Endocr Relat Cancer] 2013 Sep 11; Vol. 20 (5), pp. 725-39. Date of Electronic Publication: 2013 Sep 11 (Print Publication: 2013). |
| DOI: |
10.1530/ERC-13-0058 |
| Abstrakt: |
A chromosomal translocation results in the production of a paired box 8-peroxisome proliferator-activated receptor gamma (PAX8-PPARG) fusion protein (PPFP) in ∼35% of follicular thyroid carcinomas. To examine the role of PPFP in thyroid oncogenesis, the fusion protein was stably expressed in the non-transformed rat thyroid cell line PCCL3. PPFP conferred on PCCL3 cells the ability to invade through Matrigel and to form colonies in anchorage-independent conditions. PPFP also increased the fraction of cells with Wnt/TCF-responsive green fluorescent protein reporter gene expression. This Wnt/TCF-activated population was enriched for colony-forming and invading cells. These actions of PPFP required a functional PPARG DNA binding domain (DBD) within PPFP and were further stimulated by PPARG agonists. These data indicate that PPFP, through its PPARG DBD, induces Wnt/TCF pathway activation in a subpopulation of cells, and these cells have properties of cellular transformation including increased invasiveness and anchorage-independent growth. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
