Structural studies of an exopolysaccharide produced by Gluconacetobacter diazotrophicus Pal5.

Autor: Serrato RV; Setor Litoral, Universidade Federal do Paraná - UFPR, Rua Jaguariaíva 512, 83260-000 Matinhos, PR, Brazil. rvserrato@ufpr.br, Meneses CH, Vidal MS, Santana-Filho AP, Iacomini M, Sassaki GL, Baldani JI
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2013 Oct 15; Vol. 98 (1), pp. 1153-9. Date of Electronic Publication: 2013 Jul 18.
DOI: 10.1016/j.carbpol.2013.07.025
Abstrakt: Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium that has been found colonizing several plants. This acid-tolerant bacterium produces phytohormones that promote plant growth and is also able to grow in high-sugar concentrations. It has been demonstrated that exopolysaccharides (EPS), which are produced by strain Pal5 of G. diazotrophicus, play an important role in plant infection. We have investigated the structure of the EPS, which was produced by a strain of Pal5 grown in liquid medium containing mannitol as the sole carbon source. The results reveal an EPS with Glc, Gal, Man in a molar ratio of 6:3:1, respectively. NMR spectroscopy and chemical derivatization have revealed that the EPS structure has 4-O-substituted units of β-glucose, 3-O-substituted units of β-galactose and 2-O-substituted units of α-mannose. Glucose and galactose units linked at C6 were also found. The structure proposed herein is different from EPS produced by other species of Gluconacetobacter published to date.
(Copyright © 2013 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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