A novel promising biomolecule immobilization matrix: synthesis of functional benzimidazole containing conducting polymer and its biosensor applications.

Autor: Uzun SD; Department of Polymer Science and Technology, Middle East Technical University, 06800 Ankara, Turkey. Electronic address: demircisema@gmail.com., Unlu NA, Sendur M, Kanik FE, Timur S, Toppare L
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2013 Dec 01; Vol. 112, pp. 74-80. Date of Electronic Publication: 2013 Aug 02.
DOI: 10.1016/j.colsurfb.2013.07.049
Abstrakt: In order to construct a robust covalent binding between biomolecule and immobilization platform in biosensor preparation, a novel functional monomer 4-(4,7-di(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)benzaldehyde (BIBA) was designed and successfully synthesized. After electropolymerization of this monomer, electrochemical and spectroelectrochemical properties were investigated in detail. To fabricate the desired biosensor, glucose oxidase (GOx) was immobilized as a model enzyme on the polymer coated graphite electrode with the help of glutaraldehyde (GA). During the immobilization step, an imine bond was formed between the free amino groups of enzyme and aldehyde group of polymer. The surface characterization and morphology were investigated to confirm bioconjugation by X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM) at each step of biosensor fabrication. The optimized biosensor shows good linearity between 0.02mM and 1.20mM and a low limit of detection (LOD) of 2.29μM. Kinetic parameters Km(app) and Imax were determined as 0.94mM and 10.91μA, respectively. The biosensor was tested for human blood serum samples.
(Copyright © 2013 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: